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DTSTART;TZID=America/Los_Angeles:20260921T113000
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DTSTAMP:20260914T191617Z
CREATED:20260914T191617Z
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UID:10017053-1789990200-1789993800@live-events-ucsc.pantheonsite.io
SUMMARY:Headrick\, C. (BMEB) - The Regulation of mRNA Cleavage in Nonsense-mediated mRNA Decay: From Inside (intra-pathway determinants) Out (extra-pathway regulation)
DESCRIPTION:Nonsense-mediated mRNA decay (NMD) is a translation-coupled quality control pathway that degrades mRNAs that harbor a premature termination codon (PTC) and 5-30% of normal cellular mRNAs. The NMD field sits at a pivotal moment with recent developments that endonucleolytic cleavage is the primary mechanism of mRNA decay conserved across metazoans. Discovery of a conserved NMD cleavage mechanism sets the stage for a dissection of mRNA cleavage determinants and regulators. \nTo better understand how mRNA cleavage during NMD is regulated\, I will use degradome-seq based approaches via 5’RACE-seq (Rapid Amplification of 5’ cDNA Ends) to isolate the cleavage products of the NMD pathway. I will first focus my analysis on core NMD factors and helicase UPF1\, the binding site of NMD decay effectors\, to examine how UPF1 enzymatic activity affects mRNA cleavage transcriptome-wide (Aim 1). In parallel\, to assess extra-pathway regulation of mRNA cleavage during NMD\, I will identify endogenous negative regulators of mRNA cleavage (Aim 2). Contemporary models of NMD are deprived of the functional impact of cellular NMD inhibitors. I will access this novel functional space with an innovative application of CRISPR screening and quantitative\, high-throughput NGS\, known as ReLiC (RNA-linked CRISPR) paired with degradome analysis. \n  \nEvent Host: Camille Headrick\, Ph.D. Student\, Biomolecular Engineering & Bioinformatics  \nAdvisor: Joshua Arribere \n 
URL:https://live-events-ucsc.pantheonsite.io/event/headrick-c-bmeb-the-regulation-of-mrna-cleavage-in-nonsense-mediated-mrna-decay-from-inside-intra-pathway-determinants-out-extra-pathway-regulation/
LOCATION:Biomedical Sciences Building\, 575 McLaughlin Drive
CATEGORIES:Ph.D. Presentations
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